ABSTRACT
The purpose of this study was to assess effect of powder from Trigonella foenum-graecum seed anthropometrical and nutritional parameters of obese rats. This study was carried out on rats with initial weighing of 155-185 g. Rats of the control group have been fed with a standard food and water for 14 weeks. Rats of the experimental groups have been fed by a high-caloric diet for 14 weeks and fed by a standard chow which containing: fenugreek (2%.) During the research it was determined the main indicators of obesity such as body weight, food intake, body mass index, specific rate of body mass gain, energy intake, feed efficiency et al. Studies have shown that the addition of 2% powder from Trigonella foenum-graecum seed in food promoted weight loss and other anthropometrical and nutritional parameters.
ABSTRACT
Importance of dedicated web servers and specialized software for simulations of protein-protein interactions is well established. The purpose of our study was to examine the protein-protein interaction that occurred under physiological and stress conditions between peroxiredoxin II and the creatine kinase brain-type using protein-docking server ClusPro 2.0. To predict the particular site of amino acid docking, computer software analyzes various protein conformations and chooses the most profitable energy state, therefore selecting a number of possible combinations that would fit the correct profile. By co-immunoprecipitation assay, we demonstrated that two molecules Prx II and CKBB have interacted with further attenuation of this specific binding by pretreatment with selected stress factors. In previous study, we showed that the enzymatic activity of CKBB was recovered by different concentration ratios of Prx II. The specific binding models were generated by ClusPro 2.0 protein docking server and studied using PyMol software. It was shown that a number of amino acid residues including Lys 11, Arg 13, Ala 204, Arg 209for creatine kinase, and Asp 181, Glu 192, Lys 196, Glu 162, Gln 163 for Prx II have participated in the complex formation throughout the first ten conformations.
ABSTRACT
The purpose of this study was to assess the development of obesity and prediabetes in rats by determination of key indicators of lipid and carbohydrate metabolism and the development of insulin resistance after prolonged consumption of 10% fructose solution. This study was carried out on rats with initial weighing of 160-200 g. Rats of the control group have been fed with a standard food and water for 10 weeks. Rats of the experimental group have been fed with a standard food and 10% fructose solution instead of water ad libitum for 10 weeks. Levels of biochemical parameters were measured. The development of insulin resistance in experimental animals was determined by measuring the sensitivity of peripheral tissues to the action of insulin via insulin-tolerance test. Studies have shown that 10-week consumption of fructose solution leads to imbalance of lipid metabolism disorders and the development of imbalances in carbohydrate metabolism, as evidenced by rising blood glucose, glycosylated hemoglobin, uric acid, decreasing levels of insulin and the development of insulin resistance. Longterm consumption of 10% fructose solution may be a cause of obesity and prediabetes, which in turn can lead to the development of metabolic syndrome and type 2 diabetes mellitus.